Histopathology of Avocado Fruit Infected by Avocado Sunblotch Viroid
نویسندگان
چکیده
منابع مشابه
Application of a Highly Sensitive Avocado Sunblotch Viroid Indexing Method
In South Africa, ASBV indexing techniques such as biological indexing, polyacrylamide gel electrophoresis, radiolabeled oligonucleotide probes and DNA and RNA hybridisation with digoxigenin (DIG) labeled probes have been used over the years. Testing became increasingly more sensitive and less time consuming, but some known positives were still missed due to the low viroid titre. Our objective w...
متن کاملAlternative hammerhead structures in the self-cleavage of avocado sunblotch viroid RNAs.
The plus and minus RNAs of the 247 nt avocado sunblotch viroid (ASBV) undergo site specific RNA self-cleavage reactions in vitro. As with several other self-cleaving RNAs, we proposed hammerhead secondary structures for the sequence around the site of self-cleavage of both RNAs. We have shown previously that, during transcription of a dimeric plus ASBV RNA, a double-hammerhead structure formed ...
متن کاملRaman characterization of Avocado Sunblotch viroid and its response to external perturbations and self-cleavage
BACKGROUND Viroids are the smallest pathogens of plants. To date the structural and conformational details of the cleavage of Avocado sunblotch viroid (ASBVd) and the catalytic role of Mg2+ ions in efficient self-cleavage are of crucial interest. RESULTS We report the first Raman characterization of the structure and activity of ASBVd, for plus and minus viroid strands. Both strands exhibit a...
متن کاملSelf-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.
Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid (ASBV), prepared from tandem dimeric cDNA clones, occurs specifically at two sites in each transcript to give monomeric plus and minus species. The cleavage reaction occurs both during transcription and on incubation of purified transcripts at pH 8 and 37 degrees C in the presence of magnesium ions ...
متن کاملReplication of Avocado Sunblotch Viroid in the Cyanobacterium Nostoc Sp. PCC 7120
1Aix Marseille University, CNRS, UMR Chemistry Laboratory Bacterial 7283. 31 Chemin Joseph Aiguier 13009 Marseille Cedex 20, France 2Institute of Systematics, Evolution, Biodiversity ( ISyEB ), CNRS, MNHN , UPMC, EPHE, UPMC Sorbonne University, 57 rue Cuvier, PO Box 50, F-75005 Paris, France 3Team Enzymology of RNA, UR6, UPMC Sorbonne University, 75252 Paris Cedex 05 France 4Laboratory of Plant...
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ژورنال
عنوان ژورنال: Journal of Agricultural Science
سال: 2014
ISSN: 1916-9760,1916-9752
DOI: 10.5539/jas.v6n9p158